Formation of disulfide bond correctly and high efficiently is the key of synthesis of multiple disulfide boned-containing peptide. This article reviewes the strategy and optimization of disulfide bond formation and the method for analyzing disulfide linkage patterns in peptide. 二硫键的正确配对是富含二硫键多肽合成的关键,本文综述了含两对二硫键以上的多肽二硫键的形成策略、优化方法、以及二硫键配对方式的测定方法。
A review with 47 references on the strategy of disulfide bond formation in peptide synthesis and the methods for analyzing disulfide linkage patterns in peptides is presented. 综述了目前多肽合成领域构建2~3个二硫键的策略和对二硫键排列方式给予定位的方法。
Reduced glutathione, abbreviated as GSH, is a tri-peptide which is made up of glutamic acid, cysteine and glycine. Among these amino acids, aminoglutaminic acid 'gamma carboxyl group and cysteine join into action to form peptide linkage. 还原型谷胱甘肽(L-Glutathione)简称为GSH,是由谷氨酸、半胱氨酸和甘氨酸构成的三肽,其中谷氨酸是以γ-羧基与半胱氨酸形成肽键。